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whole brain

whole brain

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whole brain
00:19

whole brain

200623 intestine_a-sma tile-small (1)
00:33

200623 intestine_a-sma tile-small (1)

Figure legend 1.3 mm-thick cleared mouse intestine tissue labeled with α-SMA (abcam, ab5694, 1:50) at 37˚ for 4 days, followed by Goat anti-rabbit IgG (Alexa Fluor 647) secondary antibody(1:500). Image was acquired on a Nikon A1R microscope system. Movie was constructed with IMARIS image program. The tissue clearing solution was purchased from Binaree Inc.
210728-PDL1_PanCK_small (1)
00:36

210728-PDL1_PanCK_small (1)

3D Visualization 0.2 mm-thick lung cancer (H1975) tissue labeled with PCNA (1:100) and phospho-EGFR conjugated with alexa 488 (1:100) at 37˚ for 4 days, followed by Donkey Anti-Mouse IgG (Alexa Fluor ® 647) Secondary antibody for PCNA (1:200 dilution). The nuclear counter stain is DAPI (blue). PCNA (red, Abcam, ab29), phospho-EGFR conjugated with Alexa Fluor ®488 (green, Abcam, ab205827) Microscope : Nikon A1R microscope Imaging : IMARIS software.
3D bio Image of mouse liver tissue
01:00

3D bio Image of mouse liver tissue

0.2 mm-thick mouse liver tissue labelled with TNF-alpha (1:100) and alpha-SMA (1:100) at 37˚ for 3 days, followed by goat anti-hamster IgG (Alexa Fluor ® 647-red) and by donkey anti-rabbit IgG (Alexa Fluor488-green) secondary antibody (1:200 dilution), respectively. The nuclear counter stain is DAPI (blue). 3D images were reconstructed with IMARIS software.
Z-stacking images of cleared 3D dermal papilla spheroids
02:13

Z-stacking images of cleared 3D dermal papilla spheroids

Isolated human hair follicle dermal papilla cells were placed onto spheroid induction cell culture plate (commercially available at PAMCELLTM R-600, ANK Corporation, Korea) and formed spontaneously spheroids (diameter 100 um).
thy-1 video
01:00

thy-1 video

200423 intestine_tuj1(mab1195)_1
00:49

200423 intestine_tuj1(mab1195)_1

3D Visualization of Mouse Skin by Binaree
00:49

3D Visualization of Mouse Skin by Binaree

Binaree. www.binaree.com [Tissue Clearing : 3D Visualization of Mouse Skin by Binaree] Staining Green : Cell Nuclei (DAPI, false colour) Red : Blood vessel (Lectin FSD 647) Microscope Nikon A1R Confocal Laser Scanning Microscope Microscope Nikon A1R Confocal Laser
3D Visualization of whole mouse heart
00:57

3D Visualization of whole mouse heart

3D Visualization whole mouse heart stained with alpha-smooth muscle actin (red, Abcam, ab5694)
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